EVALUATION OF ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF EXTRACTS FROM THREE SPECIES OF THE GENUS Allium: A. cepa, fistulosum AND sativum GROWN IN AGRICULTURAL AREA OF DOUSSEN (WILAYA OF BISKRA)

  • Tarek BENMEDDOUR Département des sciences de la nature et de la vie, université de Biskra Laboratoire de Diversité des Ecosystèmes et Dynamique des Systèmes de Production Agricoles en Zone Arides, université de Biskra.
  • Hocine LAOUAR Laboratoire de Valorisation des Ressources Biologiques naturelles, Faculté des Sciences, Université Ferhat Abbas, Sétif 19000 Algérie.
  • AmiraAfaf BENABDI Département des sciences de la nature et de la vie, université de Biskra.
  • Safa BRAHIMI Département des sciences de la nature et de la vie, université de Biskra.

Résumé

RESUME
Les effets antibactériens et antifongiques in vitro, de trois espèces appartenant au genre Allium, sont évalués à partir des jus
frais et d’huiles essentielles de A. cepa, fistulosum et sativum. Différentes concentrations sont utilisées sur trois souches
bactériennes : Staphylococcus aureus ATCC43300, Streptococcus pneumoniae ATCC49619 et Escherichia coli ATCC25922 et
deux espèces fongiques Fusarium graminearum et Candida albicans. Le pouvoir inhibiteur semble être proportionnel à la
concentration. L’activité antibactérienne la plus élevée est obtenue par les jus frais. Nous avons noté que, pour l’activité
antifongique, Candida albicans semble être plus sensible à l’effet de l’extrait frais, alors que F. graminearum est sensible aux
huiles. L’inhibition la plus élevée est obtenue par les jus frais.
MOTS CLES: Allium fistulosum L., Allium sativum L., Allium cepa L., huile essentielle, jus frais, activité antibactérienne,
activité antifongique.
ABSTRACT
The antibacterial and antifungal effects in vitro of three species of the genus Allium were evaluated from fresh juices and
essential oils of A. cepa, fistulosum and sativum. Different concentrations are used on three bacterial strains: Staphylococcus
aureus ATCC43300, Streptococcus pneumoniae ATCC49619 and Escherichia coli ATCC25922 and two fungal species
Candida albicans and Fusarium graminearum. The inhibitory power seems to be proportional to the concentration. The highest
antibacterial activity is obtained from fresh juices. We noted that for the antifungal activity, Candida albicans seems to be more
sensitive to the effect of the fresh extract, while F. graminearum is sensitive to oils. The highest inhibition is achieved by fresh
juices.
KEY WORDS: Allium fistulosum L., Allium sativum L., Allium cepa L. essential oils, fresh juices, antibacterial, antifungal.

Références

[1] Akgül A., Kivanc M. 1988. Inhibitory effects of
selected Turkish spices and oregano components on
some foodborne fungi. International Journal of Food
Microbiology 6(3):263-268.
[2] Balestra G.M., Heydari A., Ceccarelli D., Ovidi E.,
Quattrucci A. 2009. Antibacterial effect of Allium
sativum and Ficus carica extracts on tomato bacterial
pathogens. Crop Protection 28(10):807–811.
[3] Benkeblia N. 2004. Antimicrobial activityof essential
oil extracts of various onions (Allium cepa) and garlic
(Allium sativum). Lebensm.-Wiss. u.-Technol.
37:263–268.
[4] Bernadet M. 2007. la phyto-aromathérapie pratique :
Usage thérapeutique des plantes médicinales et des
huiles essentielles. Dictionnaire thérapeutique de 530
affections courantes. Editions Dangles, 449 p.
[5] Block E., Naganathan S., Putman D., Zhao S. H. 1992.
Allium chemistry: HPLC analysis of thiosulfonates
from onion, garlic, wild garlic, leek, scallion, shallot,
elephant garlic, and Chinese chive. Uniquely high
allyl to methyl ratios in some garlic samples. Journal
of Agriculture and Food Chemistry 40:2418–2430.
[6] Buchbauer G., Jirovetz L. 1994. Aromatherapy—use
of fragrances and essential oils as
medicaments. Flavour and Fragrance journal 9(5):217-
222.
[7] Cavallito C.J., Buck J.S., Suter C.M. 1944. Allicin, the
antibacterial principle of Allium sativum. I. Isolation,
physical properties and antibacterial action. J. Am.
Chem. Soc. 66:1950–1951.
[8] Cowan M.M. 1999. Plant products as antimicrobial
agents. Clinical Microbiology Reviews 12:564-582.
[9] Cristani M., D’Arrigo M., Mandalari G., Castelli F.,
Sarpietro M.G., Micieli D., Venuti V., Bisignano G.,
Saija A., Trombetta D. 2007. Interaction of four
monoterpenes contained in essential oils with model
membranes: implications for their antibacterial
activity. J. Agric. Food Chem. 55:6300-6308.
[10] Gilbert J. and Woods S.M. 2006. Strategies and
considerations for multi-location FHB screening
nurseries. In: Ban T., Lewis J.M., Phipps E.E. (eds.).
14
2006. The global Fusarium initiative for international
collaboration: A strategic planning workshop held at
CIMMYT, El Batán, Mexico; March 14 - 17, 2006.
CIMMYT, Mexico, pp. 93-102.
[11] Goldman E.L., Green H. 2008. Practical Handbook of
Microbiology, Second Edition. CRC Press, 864 p.
[12] Grubben G. J. H. 2004. Vegetables. PROTA, 667 p.
[13] Irkin R., Korukluoglu M. 2007. Control of Aspergillus
niger with garlic, onion and leek extracts. African
Journal of Biotechnology 6(4):384-387.
[14] Kyung K. H., Kim M. H., Park M. S., Kim Y. E. 2002.
Alliinase-independent inhibition of Staphylococcus
aureus B33 by heated garlic. Journal of Food Science
67:780–785.
[15] Leroux P., Credet A. 1978. Document sur l‘étude de
l‘activité des fongicide. INRA, Versailles, France, 12
p.
[16] Liu X.C., Lu X.N., Liu Q.Z., Liu Z.L. 2014.
Evaluation of insecticidal activity of the essential oil
of Allium chinense G. Don and its major constituents
against Liposcelis bostrychophila Badonnel. Journal
of Asia-Pacific Entomology 17(4):853–856.
[17] Lucini E.I., Zunino M.P., López M.L., Zygadlo J.A.
2006. Effect of Monoterpenes on Lipid Composition
and Sclerotial Development of Sclerotium cepivorum
Berk. J. Phytopathol. 154: 441–446.
[18] Mohlenbrock R. H. 1970. Flowering Plants: Lilies to
Orchids. SIU Press, 288 p.
[19] Pizzorno J.E., Murray M.T. 2012. Textbook of
Natural Medicine. Elsevier Health Sciences, 1916 p.
[20] Ponce A.G., Fritz R., Del Valle C.E., Roura S.I. 2003.
Antimicrobial activity of essential oils on the native
microflora of organic Swiss chard. Lebensmittel-
Wissenschaft und -Technologie 36:679–684.
[21] Sévenet T., Tortora C. 1994. Plantes, molécules et
médicaments. Nathan, CNRS Editions Paris, 119 p.
[22] Shankaranarayana M. L., Raghavan B., Abraham K.
O., Natarajan C. P. 1982. Sulphur compounds in
flavours. In : Morton I. D., Macleod A. J. 1982. Food
Flavors. Elsevier Science Publishers, Amsterdam, Part
A, pp. 169-281.
[23] Sharma N., Tripathi A. 2006. Fungitoxicity of the
essential oil of Citrus sinensis on post-harvest
pathogens. World Journal of Microbiology and
Biotechnology 22(6):587-593.
[24] Soković M., Van Griensven L. J. 2006. Antimicrobial
activity of essential oils and their components against
the three major pathogens of the cultivated button
mushroom, Agaricus bisporus. European Journal of
Plant Pathology 116(3):211-224.
[25] Veldhuizen E.J.A., Hendriks H.G., Hogenkamp A.,
van Dijk A., Gaastra W., Tooten P.C., Haagsman H.P.
2006. Differential regulation of porcine beta-defensins
1 and 2 upon Salmonella infection in the intestinal
epithelial cell line IPI-2I Vet. Immunol.
Immunopathol. 114:94–102.
[26] Winkler G., Iberl B., Knobloch K. 1992. Reactivity of
Allicin and its transformation products with sulfhydryl
groups, disulfide groups and human blood. Planta
Medica 58 (Supplement Issue 1):A665.
[27] Ye C.L., Dai D.H. and Hu W.L. 2013. Antimicrobial
and antioxidant activities of the essential oil from
onion (Allium cepa L.). food control 30(1):48–53.
[28] Yin M.C., Tsao S.M. 1999. Inhibitory effect of seven
Allium plants upon three Aspergillus species. Int. J.
Food Microbiol. 49:49-56
Publiée
2015-03-16
Comment citer
BENMEDDOUR, Tarek et al. EVALUATION OF ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF EXTRACTS FROM THREE SPECIES OF THE GENUS Allium: A. cepa, fistulosum AND sativum GROWN IN AGRICULTURAL AREA OF DOUSSEN (WILAYA OF BISKRA). Courrier du Savoir, [S.l.], v. 19, mars 2015. ISSN 1112-3338. Disponible à l'adresse : >http://revues.univ-biskra.dz/index.php/cds/article/view/1198>. Date de consultation : 18 sep. 2020